Composite

Part:BBa_K571010

Designed by: Lu-Chu Ke   Group: iGEM11_TzuChiU_Formosa   (2011-10-11)


R0011+acsCD+CMCax+Ccp/pSB1C3

The biobrick is composite element, it is the combination of BBa_K571006 and BBa_K571002. The promoter binding to the upstream of acsCD, CMCax, and Ccp gene, with IPTG as the inducer, activates the genes to synthesize proteins.

The sequence of promoter, acsCD, CMCax, and Ccp is amplified by PCR, followed by enzyme digestion and ligation to bind the 4 sequences with the backbone-pSB1C3.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
    Illegal NgoMIV site found at 662
    Illegal NgoMIV site found at 1738
    Illegal NgoMIV site found at 2858
    Illegal NgoMIV site found at 5083
    Illegal NgoMIV site found at 5834
    Illegal AgeI site found at 874
    Illegal AgeI site found at 2165
    Illegal AgeI site found at 4501
    Illegal AgeI site found at 5140
    Illegal AgeI site found at 5993
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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